Extraction methods for preparation of bioactive plant extracts: A comparative study (2025)

Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity

A comparative study on the antimicrobial properties of extracts from medicinal plants obtained by two di¡erent methods was carried out.The screening of the antimicrobial activity of extracts from six plants was conducted by a disc di¡usion test against Gram-positive, -negative and fungal organisms.The most active extracts (inhibition diameter r12 mm) were assayed for the minimum inhibitory concentration and submitted to phytochemical screening by thin-layer chromatography and bioautography.The results obtained indicate that the diethyl ether extracts were the most e¤cient antimicrobial compounds.The activity was more pronounced against Gram-positive and fungal organisms than against Gram-negative bacteria. Bioautography showed that the antimicrobial activity was probably due to £avonoids and terpenes.

Comparative Study of Various Methods for Extraction of Antioxidant and Antibacterial Compounds from Plant Seeds

2012

Extracts from seeds of five different plants were prepared in water, methanol, and ethanol by employing five different methods of extraction viz. Soxhlet method, ultrasonication, extraction by continuous shaking at room temperature, and microwave assisted extraction-with and without intermittent cooling. All these extracts were compared with respect to extraction efficiency, total phenol content, total flavonoid content, antioxidant capacity, and antibacterial activity. Soxhlet method proved best in terms of high extraction efficiency, and extraction of phenolic compounds. Microwave assisted extraction with intermittent cooling (MAE), room temperature extraction by shaking (ERT), and ulrasonication assisted extraction (UAE) proved good at extracting antibacterial compounds from plant seeds. Latter also proved effective at extracting antioxidant compounds. Extraction efficiency was found to have no notable correlation with any of the parameters assayed. Methanol proved most suitable solvent for extraction of flavonoids.

Antimicrobial screening of Medicinal plants against human Pathogens-A Comparative account of two different methods of Extraction "Antimicrobial screening of Medicinal plants against human Pathogens-A Comparative account of two different methods of Extraction

Medicinal plants are part and parcel of human society to combat diseases from the dawn of civilization. The use of plants as medicine is widespread throughout the world. The herbal products today symbolize safety in contrast to the synthetics that are regarded as unsafe to human and environment. Although herbs had been priced for their medicinal, flavoring and aromatic qualities for centuries, the synthetic products of the modern age surpassed their importance, for a while. [1] However, the blind dependence on synthetics is over and people are returning to the naturals with the hope of

ffect of Extraction Methods on Bioactive Compounds of Plant Origin

Turkish Journal of Agriculture - Food Science and Technology

The use of bioactive compounds has been maintaining its significance from nutritional aspects. Due to the increasing demand for them in potential markets, researchers struggle to create new sources and improve their methods. Plant materials possess plenty and a diverse range of these compounds. However, their availability strongly depends on the extraction techniques in addition to the sampling methods and the applicability of the method to the specific parts of the plant. Thus, it is crucial to develop a common, precise way which will enable to extract all the active components regardless of their origin and their location in the plant material. Besides, the new method ought to have the highest economic value in comparison to the present applications which means that the efficiency of the extraction should be acceptable on industrial scale as well. Even though numerous methods have been improved so far, it seems to be unlikely to achieve a standardized solution with high valorizati...

Modern Extraction Methods for Preparation of Bioactive Plant Extracts

2012

Extraction is the first crucial step in preparation of plant formulations. Modern methods of extraction are effective in advancing the development of traditional herbal remedies. The development of modern sample-preparation techniques with significant advantages over conventional methods for the extraction and analysis of medicinal plants is likely to play an important role in the overall effort of ensuring availability of high-quality herbal products to consumers worldwide. Sample preparation is of utmost importance to the development of analytical methods for the analysis of constituents present in the botanicals and herbal preparations. In this article principle behind operation of various extraction methods, factors influencing method performance, research progress, strength and weakness of different extraction approaches are discussed. Emphasis is put on the methods which are solvent and energy saving, and suitable for thermolabile phytocompounds.

A Review on the Extraction Methods Use in Medicinal Plants, Principle, Strength and Limitation

Medicinal & Aromatic Plants, 2015

A Review on the Extraction Methods Use in Medicinal Plants

Medicinal plants are gaining much interest recently because their use in ethno medicine treating common disease such as cold, fever and other medicinal claims are now supported with sound scientific evidences. The study on medicinal plants started with extraction procedures that play a critical role to the extraction outcomes (e.g. yield and phytochemicals content) and also to the consequent assays performed. A wide range of technologies with different methods of extraction is available nowadays. Hence, this review aim to describe and compare the most commonly used methods based on their principle, strength and limitation to help evaluating the suitability and economic feasibility of the methods.

A review on extraction and phytochemical screening methods

Research in Pharmacy and Health Sciences, 2016

Plants are the source of different drugs belonging to various therapeutic cateogries like antidiabetics, antispasmodics, antihypertensives, anticancers, antidepressants, antimicrobials, etc. Plants are used to treat various ailments and these plants have been used by different individuals and tribals worldwide. Use of plants to treat various ailments have also been mentioned in Ayurveda. Along these lines, various researchers are involved in isolating and assessing different bioactive molecules, to be isolated from various plant sources. Isolation of bioactive molecules is not a easy task for researchers.This review gives a focus on extraction and phytochemical screening methodsalongwith their merits and demerits.

Antimicrobial screening of Medicinal plants against human Pathogens-A Comparative account of two different methods of Extraction

2012

EXTRACTION, ISOLATION AND CHARACTERIZATION OF BIOACTIVE COMPOUNDS FROM PLANTS' EXTRACTS

Natural products from medicinal plants, either as pure compounds or as standardized extracts, provide unlimited opportunities for new drug leads because of the unmatched availability of chemical diversity. Due to an increasing demand for chemical diversity in screening programs, seeking therapeutic drugs from natural products, interest particularly in edible plants has grown throughout the world. Botanicals and herbal preparations for medicinal usage contain various types of bioactive compounds. The focus of this paper is on the analytical methodologies, which include the extraction, isolation and characterization of active ingredients in botanicals and herbal preparations. The common problems and key challenges in the extraction, isolation and characterization of active ingredients in botanicals and herbal preparations are discussed. As extraction is the most important step in the analysis of constituents present in botanicals and herbal preparations, the strengths and weaknesses of different extraction techniques are discussed. The analysis of bioactive compounds present in the plant extracts involving the applications of common phytochemical screening assays, chromatographic techniques such as HPLC and, TLC as well as non-chromatographic techniques such as immunoassay and Fourier Transform Infra Red (FTIR) are discussed.